Determination of Chlorogenic Acid in Ferment Liquor of Eucommia ulmoides Oliv. Leaves by RP-HPLC-PAD

A determination method of chlorogenic acid by high performance liquid chromtograph and photodiode array detector was established. The factors such as: the chromatographic column, SymmetrySheild RP18 (3.9×300mm, 5μm), the water- methanol-acetic acid (88:12:1.2, V/V) mobile phase with 1.0ml/min flow rate, the detected wavelength (326nm), and the column temperature(25℃) were adopted. The calibration curve of chlorogenic acid was linear under the content of 2.24 ～11.2μg, with the correlation coefficient 0.9993. The method is simple, accurate and of wider linear range. The method can be adapted to control the quality of material and products that contain chlorogenic acid.