ASSEMBLING OF THE FOUR INDIVIDUAL HELICES CORRESPONDING TO THE TRANSMEMBRANE SEGMENTS (S4 IN REPEAT I-IV) OF THE SODIUM CHANNEL

Abstract A template having four cysteines with selectively detachable protecting groups was developed, which enables selective introduction of individual helices by chemical ligation between the free sulfhydryl group on the template and the chloroacetyl moiety of the helical peptide. Using the template, a protein having ion channel activity with rectification was obtained.