Development of monoclonal antibody sandwich-ELISA for determination of beer foam-active proteins

The development of a method to determine foam-active and haze-active proteins by enzyme-linked immunosorbent assays (ELISA) based on polyclonal antibodies was previously reported. Several different monoclonal antibodies against foam-active proteins in beer were developed. These monoclonal antibodies have enabled us to develop a sandwich-ELISA for the detection of foam-active proteins. Compared with our former ELISA, this new system showed the following advantages: the detection limits for the foam-active protein determined with the new ELISA system were greatly improved, and the contents of foam-active protein quantified by new ELISA system were highly correlated with the foam adhesion on beer. This system was applied to the evaluation of foam-active proteins during the brewing process.