Study on distribution of nerve fibres in the gastrointestinal tract of buffalo (Bubalus bubalis).

A study was conducted to elucidate nerve fibres and plexuses in enteric nervous system of buffalo using Bielschowsky method and by staining for Acetylcholinesterase (AchE). Tissues of twelve adult buffaloes of 1-1.5 years of age were studied in the present work. Bielschowsky silver revealed neuronal elements as dark black fibers of variable thickness and they stained light yellow to dark colour with AchE. Myenteric and submucosal plexuses had AchE positive nerve fibers. These nerve fibres extended from submucosa to mucosa and terminated on wall of crypts. Cholinergic innervations were demonstrated in the core of villi. The terminal segments of the digestive tract showed greater AchE activity.