Enzymatic characterization of rMbovP328.

Influence of temperature (A), pH values (B), divalent cations (C), and Mn2+ concentration (D) on the relative enzymatic activity of M. bovis rMbovP328. The phosphodiesterase activity of rMbovP328 was determined by HPLC analysis of c-di-AMP hydrolysis. Data shown in panels A to D are presented as the means values of three independent assays, with standard deviations indicated by error bars. (PPTX)