Antioxidant activity and constituents of propolis collected in various areas of China

Abstract Propolis is a resinous substance collected by honeybees from various plant sources. The composition of propolis depends on time, vegetation, and the area of collection. This study examined the antioxidant activity of propolis from various areas of China: Heilongjiang, Neimongol, Hebei, Shandong, Shanxi, Gansu, Henan, Hubei, Sichuan, Hunan, Yunnan and Hainan. Ethanol extracts of propolis (EEP) were prepared and evaluated for their antioxidant activities by β-carotene bleaching, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical-scavenging, and 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation decolorization assays. Furthermore, the major constituents in EEP were identified by high-performance liquid chromatography (HPLC) analysis with a photodiode array (PDA) and mass spectrometric (MS) detection, and each component was quantitatively analyzed. All propolis samples except that from Yunnan had relatively strong antioxidant activity accompanied by high total polyphenol contents. Propolis with strong antioxidant activity contained large amounts of antioxidative compounds, such as caffeic acid, ferulic acid and caffeic acid phenethyl ester. On the other hand, propolis from Yunnan and Hainan had compounds not present in propolis from other areas.