Isolation and characterization of antiviral and ribosome inactivating protein from the endophytic fungi Alternaria sp from Viscum album using MADLI-TOF-MS and their antibacterial activity

Five different endophytic fungi was isolated and identified from the various parts of Viscum album, protein isolated from the endophytic fungi Alternaria sp and Viscum album was identified as lectin based on haemagglutination assay, isolated lectin was blood group specific lectin agglutinating A+ve erythrocytes. SDSPAGE showed the molecular weight of 64 kDa protein was observed in endophytic Alternaria sp similar to Viscum album, PAS staining of NATIVE PAGE confirms the presence of lectin, MALDI-TOF-MS analysis of endophytic fungal lectin and MASCOT peptide mass finger printing reported that endophytic fungal lectin belongs to antiviral protein and ribosome inactivating protein family, observed peptide sequence K.NGALPKPLELK, identified as a conserved sequence pattern in the ribosome inactivating protein (RIPL1_PHYDI) of Phytolacca dioica (Bella sombra) tree and antiviral protein(RIPS_PHYAM) of Phytolacca americana (American pokeweed), this confirms that endophytic fungi Alternaria sp capable of producing lectin having antiviral and anticancer activity. Endophytic fungal lectin showed in vitro antibacterial activity on human pathogenic bacteria, maximum inhibition of 9 mm in Pseudomonas sp, 11 mm inhibition in Salmonella sp was observed from endophytic lectin extract.