HA-PIPP(上标 H557A)对小鼠受精卵卵裂的影响

Objective To study the regulative role of PIPP(superscript H557A) (Proline-rich Inositol Polyphosphate 5-Phosphatase Histidine 557 Alanine) on cleavage of mouse fertilized eggs. Methods With respective transfection of HA-PIPP(superscript H557A), HA-PIPP or HA vector to mouse fertilized eggs to detect the division rate by direct counting under dissect microscope and indirect immunofluorescence analysis under fluorescence microscope. Results Contrary effects of HA-PIPP(superscript H557A) with HA-PIPP on cell cleavage were observed during mitotic course of mouse fertilized eggs. The division rate was significant, compared with each other (P＜0.05). Conclusion PIPP(superscript H557A) may interruptted cell cleavage of mouse fertilized eggs by regulating the activity of PIPP.