Potential effects of suspended TiO2 nanoparticles on activated sludge floc properties in membrane bioreactors

Abstract With the rapid development and application of consumer products containing nanoparticles (NPs), especially titanium dioxide (TiO 2 ) NPs, the potential effects of suspended NPs on wastewater treatment has been a concern over the recent years. This study investigated the potential effects of suspended TiO 2 NPs on activated sludge flocculation properties in a membrane bioreactor (MBR). Results showed that suspended TiO 2 NPs inhibited the viability of activated sludge flocs, and led to bacterial protein secretion for bacterial protection, causing an overall protein increase of soluble microbial products. Suspended TiO 2 NPs also destabilized the activated sludge floc structure and reduced flocculation capacity of flocs, causing an over production of organic matter and resulting in a floc size decrease of over 50%. Suspended TiO 2 NPs also caused a change in the phylogenetic distribution of bacterial community. Whereby, the dominant species in activated sludge was replaced from Comamonadaceae to Thiotrichaceae in 50 mg/L suspended TiO 2 NPs.