Gas Chromatography of D-Glucaric Acid and Its Lactones, and Application to Measuring Their Transformation

A simple and reliable analytical method for D-glucaric acid, D-glucaro-1, 4-lactone and-6, 3-lactone was established; the sample solution was freeze-dried, trimethylsilylated and applied to a gas chromatograph.The transformation of D-glucaro-1, 4-lactone into D-glucaric acid and D-glucaro-6, 3-lactone at physiological pH and temperature was traced by this method. Similarly, the transformations of L-gulono-γ-lactone into L-gulonic acid and of D-glucono-γ-lactone into D-gluconic acid and D-glucono-δ-lactone were traced.