Estudio comparativo in vitro del efecto antibacteriano entre tres sustancias utilizadas como medicamento intraconducto contra cepas de enterococus faecalis.

Enterococcus faecalis which is one of the microorganisms that cause problems and even failure during the endodontic procedures has the ability to adhere to tissues, form biofilms and great resistance to antibiotics, allowing it to survive endodontic therapy and cause post-treatment diseases. The aim of this research was to determine the antimicrobial effectiveness of calcium hydroxide with 2% chlorhexidine, the triple antibiotic paste of amoxicillin, ciprofloxacin and metronidazole and the ethanol extract of 50% propolis against Enterococcus faecalis. An in vitro study was carried out by the diffusion method on Muller Hinton agar inoculated with the bacteria under study.The total sample was divided into 3 groups: G1: Calcium hydroxide + 2% chlorhexidine, as negative control distilled water was used and as positive control ampicillin solution was included. G2: 30% triple antibiotic paste, as negative control Propylene glycol and as positive control ampicillin solution. G3: Ethanol extract of propolis 50%, as negative control ethanol and positive control ampicillin solution. In each petri dish 3 wells were made, where 80ul of the drugs and of each positive and negative control were placed, later the boxes were incubated for 24 hours at 37oC and finally, the measurement of the inhibition zones was compared at 24 hours, being these directly proportional to the antimicrobial activity. The highest antimicrobial activity was given by the triple antibiotic paste, which presented an average halo of 43 mm, compared to calcium hydroxide of 20 mm and the ethanolic extract of propolis of 10 mm The growth of the inhibitory halo of the triple antibiotic paste was significantly higher than the growth of calcium hydroxide + chlorhexidine at 2% and the ethanolic extract of propolis at 50% (P = 0.008). Keywords: Enterococcus faecalis; Endodontics Antibiogram.