Atypical CTSK transcripts and ARNT transcription read-through into CTSK.
2005
The aryl hydrocarbon receptor nuclear translocator (ARNT) and cathepsin K
(CTSK) genes lie in a tandem head-to-tail arrangement on human chromosome 1. The two
genes are in extremely close proximity; the usual CTSK transcription start site is
less than 1.4 kb downstream of the end of the longest reported ARNT transcript.
By generating an RT-PCR product that overlaps both the 3′ end of ARNT and
the 5′ end of CTSK, we show that ARNT transcripts may extend through the
ARNT–CTSK intergenic region and progress into the CTSK gene.
Furthermore, by using quantitative RT-PCR from several tissues to detect the ARNT expression
signature in CTSK introns, we show that ARNT transcripts can read through into
CTSK as far as CTSK intron 3, extending approximately 3.7 kb downstream of the
end of the longest previously described ARNT mRNA. Given that ARNT and
CTSK are expressed in an overlapping range of tissues, ARNT read-through may have a
negative impact on CTSK transcript levels by interfering with CTSK expression.
We also present evidence for novel CTSK transcripts following sequence analysis
of CTSK-derived ESTs and RT-PCR products. These transcripts show alternate 5′
splicing and or 5′ extension and are sometimes initiated from a cryptic alternative
promoter which is upstream of the known CTSK promoter and possibly in the 3′
UTR of ARNT.
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