Determination of Neutral Saccharide and Uronic Acid in Polysaccharides of Ganoderma Applanatum

Objective:To determined the contents of neutral saccharide and uronic acid in polysaccharide of Ganoderma applanatum.Methods:The contents of neutral saccharide were determined by spectrophotometer with glucose as standard,the contents of uronic acid were determined by spectrophotometer with glucuronic acid as standard,the affecting between neutral saccharide and uronic acid was investigated by simulative methods.Results:Th analyses were simple,accurate and could be used for controlling the quality of polysaccharides of Ganoderma applanatum.Conclusion:Flavone affects the dederminaton of hydroxybenzene acid,modify coefficients is 0.91.