Sequence length and error analysis of Sequenase and automated Taq cycle sequencing methods.

We have examined DNA sequence error as a function of length using both a manual method of performing reactions with Sequenase® and an automated Taq cycle sequencing method. DNA fragments from both methods were separated and analyzed on a sequencer. To determine the sequence of a cosmid insert (35.3 kb), 379 sequences were obtained from a manual Sequenase method, and 354 sequences were obtained from a Taq cycle sequencing method as performed on an automated robotic workstation and sequenced on an automated fluorescent sequencer