Mechanism of Notch and Sox2 in Kras driven lung adenocarcinoma in type II cells

sequencing space/sample, and d) frequency of polymerase error at the nucleotide site bearing the mutation. In targeted NGS, synthetic competitive IS control for stochastic sampling at input of both target into library preparation and of target library product into sequencer, enable reduced sequencing space requirement, and control for qualitative errors generated during library preparation and sequencing. These controls enable accurate clinical diagnostic reporting of confidence limits and limit of detection for copy number measurement, and reduce sequencing space required for analysis.