Nucleoside derivative and preparation method thereof

The invention aims to provide a synthetic method for obtaining a fluorescence nucleoside derivative by adopting 7-diethylin-4-methylcoumarin and 5-iodouridine as raw materials and reacting with a palladium catalyst. The synthetic method is characterized by comprising the following steps of (1) stirring the 7-diethylin-4-methylcoumarin and N-bromo-succinimide at the temperature ranging from 30 to 40 DEG C for 7 hours, and treating to obtain an intermediate product;(2) stirring the product obtained in the last step and a catalyst at the temperature ranging from 65 to 70 DEG C for 4 to 6 hours, cooling to the room temperature, adding a certain amount of potassium carbonate, then stirring for 3 hours, and treating to obtain coumarin acetylide; (3) dissolving the product obtained in the last step, the 5-iodouridine, a certain amount of palladium catalyst and cuprous iodide into N,N-dimethylformamide, reacting at the temperature ranging from 65 to 70 DEG C for 2 to 5 hours, and separating and purifying to obtain a final product. The product obtained through the invention has a favorable fluorescence characteristic, can be used as a fluorescence nucleoside probe, and has a better application prospect in the fields such as biological chemistry and medicines.