Effect of hematopoietic and lymphoid cells on the stromal CFU colony formation in rabbit bone marrow cell cultures

Guinea pig and rabbit bone marrow and splenic cells with increasing cellular density suppress in vitro formation of fibroblast colonies by rabbit bone marrow clonogenic stromal cells. At the same time guinea pig bone marrow and splenic cells produce a stimulating effect on guinea pig bone marrow CFUf, but they are inhibited by rabbit splenocytes, although to a lesser extent than rabbit bone marrow CFUf. Rabbit blood platelets stimulate the growth of bone marrow CFUf of these animals. However, introduction into the culture of rabbit bone marrow cells combined with platelets eliminates the latter's growth-stimulating effect on stromal clonogenic cells of rabbit bone marrow. The results obtained have evidenced that guinea pig and rabbit bone marrow and lymphoid cell populations contain cells both stimulating and inhibiting CFUf proliferation, and that CFUf of varying animal species have different sensitivity to growth-stimulating and growth-inhibiting effects.