Optimization of medium components and culture conditions of algicidal actinomycetes BS01

A strain of actinomycetes BS01 capable of lysing the toxic dinoflagellate Alexandrium tamarense was isolated from mangrove area of Zhangjiangkou in Fujian province.The medium components and cultural conditions for algicidal activity of actinomycete strain BS01 were optimized by single-factor and uniform tests in shake-flask.The initial pH,fer-mentation temperature,fermentation time,and inoculum were optimized through single-factor tests.Results showed that BS01 exhibited the best algicidal activity by using soluble starch as carbon source,sodium nitrate as nitrogen source,initial pH at 7.7,temperature at 27.2℃,and shaking at 150r.min?1 in 100mL of 250mL conical flask for 48h.The optimal fer-mentation parameters of BS01 were: soluble starch 20g.L?1,sodium nitrate 0.5g.L?1,pH value 7.7,and temperature 27.2℃ by using uniform design experiments.