Variation in the Frequency of Micronucleated Hepatocytes Following Time Course Treatment in the Mouse : Liver Micronucleus Assay of Partially Hepatectomized Tissues

The time-course pattern of the frequency of micronucleated hepatocytes in vivo after partial hepatectomy (PH) was studied in mice using N-nitrosodimethylamine (DMN), N-nitrosodiethylamine (DEN), and 1, 2-dimethylhydrazine (DMH), which are rodent liver carcinogens with potent clastogenic activity in the liver. With all chemicals, production of micronucleated hepatocytes was not clearly observed at 3 d after PH, but was clear 4 or 5 d after PH. We propose that it is preferable to perform a preliminary assay prior to the main assay when estimating the clastogenic potential of certain chemicals towards hepatocytes in vivo.