Initial results of LC-MAP: An institutional program to routinely profile tumor specimens for the presence of mutations in targetable pathways in all patients with lung adenocarcinoma.

7009 Background: Mutated oncogenes underlie the behavior of lung adenocarcinomas and can serve as targets for therapy. Determining the presence of these molecular abnormalities can direct the care of individual patients, qualify them for clinical trials, and aid research. To acquire this information on as many patients as feasible, in JAN 2009 our multidisciplinary Disease Management Team began a program (the Lung Cancer Mutation Analysis Project - LC-MAP), to prospectively detect all recurrent mutations in EGFR, KRAS, BRAF, HER2, PIK3CA, MEK1 and AKT1 and the EML4-ALK fusion gene in all patients diagnosed with lung adenocarcinoma with sufficient tissue. Methods: Patients sign an institutional consent to permit the use of previously obtained (“leftover”) tissue for mutation profiling after standard morphologic and molecular diagnostic studies are complete. After PCR-based testing for EGFR exon 19 deletions and L858R, KRAS mutations are determined by direct sequencing, then remaining DNA is studied in a mu...