Linear scalability of virus production in the integrity ® iCELLis ® single-use fixed-bed bioreactors from bench to industrial scale
2013
In order to maximize cell growth within a compact space and retain cells for easy medium exchange, the iCELLis bioreactors from ATMI LifeSciences contain macro-carriers trapped in a fixed-bed, creating a 3-D matrix within which cells adhere and replicate. These bioreactors also enable precise temperature, pH and dissolved oxygen control which cannot be done in 2-D cultures.
The iCELLis technology can be used at small and large scales with straightforward process scale-up, easy single-use operations and minimal space requirement.
Here we present a summary of adherent cell process development in iCELLis bioreactors, including:
• HEK 293 cell expansion for production of adenovirus
• MVA virus production in CEF cells
• Bovine Herpes Virus production in MDBK cells
• Recombinant Adeno-Associated Virus in A549 cells
• Adenovirus production in A549 cells
• Influenza virus production in Vero cells
• Paramyxovirus production in Vero cells
• Undisclosed lytic virus in Vero cells
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