Linear scalability of virus production in the integrity ® iCELLis ® single-use fixed-bed bioreactors from bench to industrial scale

In order to maximize cell growth within a compact space and retain cells for easy medium exchange, the iCELLis bioreactors from ATMI LifeSciences contain macro-carriers trapped in a fixed-bed, creating a 3-D matrix within which cells adhere and replicate. These bioreactors also enable precise temperature, pH and dissolved oxygen control which cannot be done in 2-D cultures. The iCELLis technology can be used at small and large scales with straightforward process scale-up, easy single-use operations and minimal space requirement. Here we present a summary of adherent cell process development in iCELLis bioreactors, including: • HEK 293 cell expansion for production of adenovirus • MVA virus production in CEF cells • Bovine Herpes Virus production in MDBK cells • Recombinant Adeno-Associated Virus in A549 cells • Adenovirus production in A549 cells • Influenza virus production in Vero cells • Paramyxovirus production in Vero cells • Undisclosed lytic virus in Vero cells