Polimorfismo da glutationiona-S-transferase P1 e marcadores de estresse oxidativo em mulheres infectadas por papilomavírus humano. | Glutathione-S-transferase P1 polymorphism and oxidative stress markers in women infected by human papilomavirus.

Objetivo : analisar o polimorfismo genetico de GSTP1 localizado no cromossomo 11.q13.2, exon 5 (Ile105Val) e associar a marcadores de estresse oxidativo e infeccao por HPV. Metodos : o DNA foi extraido de raspados cervicais para identificacao do HPV por PCR e genotipagem utilizando HPV-Screening PapilloCheck® e de sangue total para analise do polimorfismo por RFLP-PCR. O estresse oxidativo foi analisado atraves dos niveis sericos das especies reativas ao acido tiobarbiturico, grupos carbonil, grupos tiol e atividade da catalase. Resultados : 75% das mulheres HPV positivas apresentaram infeccoes simples e os tipos virais 16 e 45 foram mais frequentes. 56,25% das mulheres apresentaram o genotipo variante Ile105Val GSTP1 (Ile/Val ou Val/Val) com prevalencia de heterozigotos (43,75%). Entre as mulheres HPV positivas, a presenca do variante alelico levou a um maior risco de desenvolver lesoes cervicais (OR = 29,44). Mulheres infectadas com HPV (especialmente de alto risco) apresentaram menor atividade da catalase e aumento dos grupos carbonila. Considerando a presenca dos genotipos variantes Ile/Val e Val/Val, as mulheres infectadas apresentaram aumento dos marcadores de estresse oxidativo e as mulheres HPV negativas apresentaram diminuicao da capacidade antioxidante. Conclusao : estes achados sugerem uma possivel influencia do genotipo variante GSTP1 no aumento do estresse oxidativo e suscetibilidade a lesoes cervicais em mulheres infectadas com HPV. Palavras-chave : gstp1; especies reativas de oxigenio; hpv; lesao cervical. ----------------------------------------------------------------------------------------- Abstract Objective : to analyze the genetic polymorphism of GSTP1 located on chromosome 11q13.2 exon 5 (Ile105Val) and its association with oxidative stress markers and HPV infection. Methods : DNA were extracted from cervical scrapes for HPV identification by PCR, genotyping using HPV-Screening PapilloCheck®, and from whole blood for analysis of polymorphisms by RFLP-PCR. Oxidative stress was analyzed by measuring the serum levels of thiobarbituric acid reactive species, carbonyl groups, thiol groups, and catalase activity. Results : of all HPV-positive women, 75% had simple infections. Viral types 16 and 45 were the most frequent. A total of 56.25% of women had variant Ile105Val GSTP1 genotype (Ile/Val or Val/Val) with a heterozygous prevalence (43.75%). Among the HPV-positive women, the presence of the allelic variant led to a higher risk of developing cervical lesions (OR = 29.44). Women infected with HPV (especially the high-risk type) showed lower catalase activity and an increase in carbonyl groups. Considering the presence of variant genotypes Ile/Val and Val/Val, the infected women presented an increase in some oxidative stress markers, and HPV-negative women showed a decreased antioxidant capacity. Conclusion : these results indicate the potential influence of variant GSTP1 genotype on increased oxidative stress and susceptibility to cervical lesions in women infected with HPV. Keywords : gstp1; reactive oxygen species; HPV; cervical injury.