Method for preparing quantum dots with nuclear shell structures, fluorescent nanometer probe for target tumor markers GPC-3 and method for preparing fluorescent nanometer probe

The invention provides a method for preparing quantum dots with nuclear shell structures, a fluorescent nanometer probe for target tumor markers GPC-3 and a method for preparing the fluorescent nanometer probe. The method for preparing the fluorescent nanometer probe includes preparing the quantum dots with the nuclear shell structures and ZnS as shells; activating carboxyl on the surfaces of the quantum dots with the nuclear shell structures and the ZnS as the shells by the aid of carbodiimide (EDC) and N-hydroxy succinimide (NHS); adding monoclonal antibodies GC33 to the quantum dots to enable the monoclonal antibodies and the activated carboxyl on the surfaces of the quantum dots with the nuclear shell structures and the ZnS as the shells to form amido bonds; modifying the surfaces of the quantum dots by the aid of the monoclonal antibodies GC33 (IgG2a, k) to obtain the nanometer probe (GC-QDs). The methods and the fluorescent nanometer probe have the advantages that the surfaces of the quantum dots are modified by the monoclonal antibodies GC-33 to obtain the nanometer probe with high specificity and high target ability, influence of biological tissues on fluorescent interference can be overcome, accordingly, in-situ and in-vivo labeling and imaging on liver cancer can be implemented, and experimental bases and theoretical research can be provided for clinical application of the fluorescent quantum dots.