Extraction and Purification Technologies of Total Flavonoids from Aconitum tanguticum

Objective: To optimize the extraction and purification technologies of total flavonoids from Aconitum tanguticum whole plant.Methods: With the content of total flavonoids as index,the optimum extraction conditions for the concentration,volume of alcohol,extracting time and times were selected by orthogonal optimized; Comparing the adsorption quantity( mg /g) and resolution( %),four kinds of macroporous adsorption resins including D101,AB-8,X-5 and XAD-16 were investigated for the enrichment ability of total flavonoids from Aconitum tanguticum; Concentration and pH value of sample,sampling amount,elution solvent and loading and elution velocity for the optimum adsorption resin were determined.Results: The content of total flavonoids in Aconitum tanguticum was about4.39%; The optimum extraction technique was 70% alcohol reflux extraction for three times,each time for one hour,the ratio of material and liquid was 1∶ 10( w /v); The optimum purification technology was: using XAD-16 macroporous resin,the initial concentration of total flavonoids of Aconitum tanguticum was 8 mg /mL,the sampling amount was 112 mg /g dry resin,the pH value was 5,the loading velocity was 3 mL /min,the elution solvent was 70% ethanol and the elution velocity was 5 mL /min.Under the optimum conditions,the average content of total flavonoids was raised from 4.39% to 46.19%.Conclusion: The optimum extraction and purification technologies for total flavonoids of Aconitum tanguticum were suitable for industrial production for its simplicity and responsibility.