Analysis of Hinokitiol in Antimicrobial/deodorant Processed Textiles

The analytical method for identifying hinokitiol (HN) in antimicrobial/deodorant processed textiles was examined by high performance liquid chromatography (HPLC) with a photo diode array detector. The detection limit at 240nm wavelength was 3ng as S/N=5. First, the presence of HN in hiba oil was determined, and then the extraction method from hiba oil filled microcapusules was examined. Second, HN in the hiba oil filled microcapusules which adhered to the cloth was analyzed. As a result, HN was surely found in the cloth immediately after processing. To analyze the level of HN in antimicrobial/deodorant processed textiles, HN was extracted from samples with methanol under reflux at 70°C. To remove the polar materials, 20mM phosphate buffer (pH=2.4) was added to the extract, and HN was then reextracted into cyclohexane from the methanolic phase. After the extract was concentrated, it was applied to a C18 cartridge column, HN was then eluted with methanol. After concentration, the elute was applied to the HPLC. Although good recoveries of HN were developed in each operating step, the overall recoveries from textiles fortified at 10μg and 100μg were only 10∼14% and 40∼55%, respectively. Probably, the cause of such low recoveries was due to the photolysis and sublimation ability of HN. Therefore, quantitative analysis of HN was difficult using this method, but qualitative analysis was possible above the range of 10μg. Five brands of antimicrobial/deodorant processed textiles using HN were analyzed by this method, however HN was not detected in any of the samples. Consequently, it is doubtful that the antimicrobial effect of those products was dependent on the activity of HN.