Bioaugmentation to enhance anaerobic digestion of food waste: Dosage, frequency and economic analysis.

Abstract This study investigated whether bioaugmentation can improve the anaerobic digestion (AD) performance of food waste (FW), as well as the effects of addition dosage and frequency on the bioaugmentation’s performance and economic feasibility. The findings demonstrated that all the bioaugmented digesters, regardless of dosage and frequency, performed more effectively in biogas production than the non-bioaugmentation control. Furthermore, relatively higher dosages or frequencies increased AD performance. Introducing 0.25 g L-1 d-1 of bioaugmentation seed every three days increased OLR and volumetric biogas production 8-fold and 12-fold, respectively, compared to the non-bioaugmentation control. Whole-genome sequencing analysis showed that bioaugmentation enhanced the population of the acetoclastic Methanothrix (belong to the order Methanosarcinales). Moreover, high abundance of Methanothrix (exceeding 80%) contributed to a better AD performance. Economic analysis of an up-scale biogas plant suggested that an appropriate bioaugmentation process increased income, thus increasing the profit to 3696 CNY d-1 if treated at 21t FW.