Application of multi-functional PAP pen in cell climbing immunohistochemical detection

The invention discloses an application of a multi-functional PAP pen in cell climbing immunohistochemical detection. The application comprises the following steps: inoculating cells in a glass slide-type culture dish for enabling cells to grow on the glass slide-type culture dish; after cell culture, conducting immunohistochemical dyeing identification. The glass slide-type culture dish is provided with a culture dish body and a culture dish cover, wherein the culture dish body comprises a single-pore chamber model and a porous chamber model; the area of each pore chamber is greater than that of the glass slide; the bottom of the culture dish body can be directly used as the glass slide; a rectangular streak line groove, the size of which is corresponding to that of the glass slide, is formed in the outer side surfaces of the bottom of the culture dish body; the bottom of the culture dish body can be pulled out of the rectangular streak line groove under the action of external force. The application is suitable for various immunohistochemical dyeing experiments on a glass slice, can remarkably reduce antibodies and the reagent dosage, avoids liquid flow and diffusion in dyeing, improves the operation speed, and is particularly suitable for large-scale large-sample and multi-group immunohistochemical dyeing in experimental study of cell growing on the glass slide-type culture dish or cell smearing.