A MAGNETIC-BEAD-BASED IMMUNOASSAY FOR RAPID PURIFICA- TION AND DETECTION OF INFLUENZA VIRUSES UTILIZING SUCTION- TYPE MICROFLUIDIC SYSTEMS

The study presents a magnetic-bead-based, suction-type microfluidic system for rapid purification and detection of influenza viral particles from clinical serum samples. Target influenza viral particles in the clinical sample can be first captured by the antibody-conjugated magnetic beads, followed by isolating the viral particles from the bio-samples with the incorporation of a permanent magnet and a suction-type microfluidic control module. Then, another specific secondary developing antibodies labeled with fluorescent dyes were employed to recognize the surface antigen of the virusbound magnetic complexes. Finally, the fluorescent-labeled magnetic complexes can be then detected and analyzed by utilizing an optical detection module. The entire process can be performed automatically within in 30 minutes. The development of the integrated microfluidic chip may provide a promising platform for fast diagnosis of influenza infection.