Super-resolution imaging methods based on fluorescence resonance energy transfer

The present invention discloses a super-resolution imaging method based on fluorescence resonance energy transfer, the super-resolution imaging method comprising the steps of: 1) a fluorescent probe labeled with a specimen sample to be high FRET efficiency, the high efficiency of the phosphor FRET FRET probe is labeled with a molecule of said FRET molecule comprising a first fluorophore (donor) and a second fluorophore (acceptor), the first fluorophore fluorescence resonance occurs can be the second fluorophore energy transfer (FRET); 2) can make use of the excitation light intensity in step 1) of the molecule FRET fluorescence resonance energy transfer excitation light threshold, the laser scanning confocal microscope imaging. The present invention is based on fluorescence resonance energy transfer saturated super resolution technology in the conventional confocal laser microscope to achieve a super-resolution image of a biological sample, which method high resolution.