Leukocyte Profil of Koi Fish (Cyprinus carpio) Which Infested by Ichthyophthirius multifiliis on The Different Infestation Degree With Cohabitation Methode

Koi fish (Cyprinus carpio) is one type of ornamental fresh water, which much liked because of its beauty  such as have shape, colour and beautiful patern, so many people depend on  their  life from culturing and marketing koi  fish. The main obstacle in koi fish culture is disease attack. The disease caused by interaction between host, pathogen  and environment  (Fisheries and Marine Department, 2005). Ichthyophthirius multifiliis (I. multifiliis) is one of parasite which can cause fish ichtyopthiriasis. According  Durborow (1998) said, that clinical sign of koi fish who affected by I. multifiliis had a white spot. I. multifiliis is a  parasite that eat blood cells (Dogiel et al., 1970). According Macdonald et al., (2001) in Saptanto (2004) explain, that  parasite infestation also could increase eosinophile. Total of monocyte increases if there are a foreign substantion in  tissue or blood circulation and neutrophile are phagocyte which could migration in the other tissue to eat bacteria  (Moyle and Chech, 2004). Meyer and Harvey  (1998)  in Salasia  (2001) assert,  that blood  test also useful  to help disease  diagnose, examining  immune  system and  to determine  fish health  status. The method was used in this field research is experimental method by cohabitation method. This research is  expected  to provide  information and knowledge about  leucocyte component which  infested by  I. multifiliis at different  infestation level with cohabitation method. This research was done July 26, 2010 until December 31, 2010 at Klepon  Village, District of Garum, Blitar, East Java and in the Laboratory of Education Faculty of Fisheries and Marine  Fisheries, Airlangga University, Surabaya. I. multifiliis infestation level are classified based on the total of I. multifiliis who infestation koi fish total of low 1–5 parasites/slide, medium 6–10 parasites/slide, High more than 11 parasites/slide (Margolis et al., 1982 in Akter dkk., 2007). The result of research showed that chages of leucocyte blood profil of the amount the total each leucocyte component (lymphocyte, neutrophile, monocyte, eosinophile) koi fish who  infested by I. multifiliis,  there  is a declining in average percentage of total lymphocyte in low infestation 86.20%, medium infestation 79.40% and heavy  infestation 75.20%. The average percentages of  total monocyte was  increasing  in  low  infestation 5.40%, medium infestation 5.60% and heavy infestation 6.60%. The average percentages of total neutrophile also increasing in low infestation 6.40%, medium  infestation 11.20% and heavy  infestation 11.60%. The average percentages of total eosinophile also increasing in low infestation 2.00%, medium infestation 3.60% and heavy infestation 5.60%. water quality measured during  research,  the average of  temperature 24-26 C and pH cohabitation 8.