Phthalates in Albumin from Human Serum: Implications for Assisted Reproductive Technology

Abstract Albumin, a vital protein in cell culture systems, is derived from whole blood or blood products. The culture of human gametes and developing embryos for assisted reproduction (ART) uses albumin of human origin. Human serum albumin (HSA) is derived from expired blood obtained from blood banks. This blood has been stored in polyvinyl chloride bags made clear and flexible with di-2-ethylhexyl phthalate (DEHP). But DEHP can leach from the bags into stored blood and co-fractionate with HSA during albumin isolation. DEHP and its metabolite mono-ethylhexyl phthalate (MEHP), are known endocrine disruptors that are reported to have negative effects when directly supplemented in media for IVF using gametes from a variety of animals. Therefore, the contamination of ART media with DEHP and MEHP through HSA supplementation may have effects on the outcomes of ART procedures. While the embryology laboratory is strictly monitored to prevent a wide variety of contamination, phthalate contamination of HSA has not been broadly examined. This review outlines the function of HSA in ART procedures and the production of HSA from whole blood. Finally, the review highlights the effects of acute phthalate exposures on gametes during in vitro procedures.