HPLC ANALYSIS OF VITEXIN AND FINGERPRINT OF VITEX NEGUNDO L.

High Performance Liquid chromatography was done for quantification of vitexin and for examining the fingerprint of Vitex negundo methanol leaf extract. The analysis was carried out using Lichrosorb C-18 column at 25°C, with methanol: water as mobile phase. The flow rate was 1.0 ml/min and the detection was at 210 nm. The amount of vitexin present in V. negundo collected from five different locations of Western Ghats ranged from 22.91 to 83.69 µg/ml. Although the amount of the marker flavonoid (vitexin) was different, HPLC fingerprints of all samples were well conserved. The highest amount of vitexin was found in Karaiyar accession and was considered as superior genotype. The compiled data will be useful for taxonomy, to improve the quality control and authenticity testing of phytopharmaceuticals containing V. negundo extracts.