A method of producing a recombinant adeno-associated virus, suitable means for this purpose, as well as use for the preparation of a medicament

A method of producing a recombinant adeno-associated virus (rAAV) in which a in a suitable cell or more helper constructs containing nucleic acid sequences encoding (for at least a Rep protein and / or for the Cap proteins (the first construct) as well as a vector construct second construct ) containing one or more heterologous to AAV nucleic acids are flanked by ITR sequences are introduced, characterized in that the one or more helper constructs and the vector construct offset in time and introduced without intermediate selection in the appropriate cell.