Generation of induced pluripotent stem cells from peripheral blood CD34 + hematopoietic progenitors of a 31 year old healthy woman

Abstract The MUi019-A human induced pluripotent stem cell line was generated from peripheral blood CD34 + hematopoietic progenitors of a healthy woman using a non-integrative reprogramming method. Episomal vectors carrying reprogramming factors OCT4 , SOX2 , KLF4 , L-MYC , LIN28 , and shRNA of TP53 and EBNA-1 were delivered using electroporation. The iPSC line can be used as a control in studying disease mechanisms. Furthermore, gene editing approaches can be used to introduce specific mutations into the MUi019-A to model disease while the cell type affected by the disease is inaccessible.