Impact of Ca~(2+) Concentration in Bone Marrow Mononuclear Cells with Busuishengxue Granule in Chronic Aplastic Aaenia Patients and the Observation of It in Different Blood Diseases

Objective:To observe the change of Ca2+ concentration in bone marrow mononuclear cells(BMMC)in chronic aplastic anemia(CAA),exploring the possible mechanism of Busui Shengxue particles on CAA.To explore diagnosis and treatment ideas by observing the Ca2+ concentration in different blood diseases.Method: Extraction separation the CAA patients' BMMC.Use fluo3 AM staining by flow cytometry detection of intracellular calcium concentration.Detect the Ca2+ concentration in CAA,Myelodysplastic Syndrome(MDS),Idiopathic Thrombocytopenic Purpura(ITP),Iron-Deficiency Anemia(IDA) and compare them.Results: CAA patients' BMMC intracellular Ca2+ concentrations were significantly higher than those in the normal group.After Busui Shengxue particles treatment,patients in each group of intracellular Ca2+ concentration declined,Busui Shengxue particle group was better than the control group,but still higher than the normal group.MDS and CAA was higher than others.ITP,IDA and normal group were no significant difference.Conclusion: The concentration of BMMC intracellular Ca2+ rise is an important symbol of CAA disease situation.The Busui Shengxue particles can regulate Ca2 + release in BMMC,which may be one mechanism of the treatment of CAA.MDS,CAA,CITP,IDA's Ca2+ concentrations have significant differences,which may be related to the disease's degree of malignancy and mechanism.