The Screen of Interaction Protein with RBP Using Two-hybrid System

On the basis of having constructed inducing fusion vector: pGBKT7-RBP, pretransformed human kidney cDNA library PACT2 and pGBKT7-RBP were co-expressed. After libraries screening and nutritional selection as well as X-α-gal analysis, three protein sequence of putative interaction were obtained. And complete sequences were cloned with PCR application from human testis epidermis cDNA library.