Quantification of opioid-binding sites in the ewe hypothalamus

Opioid-binding sites were quantified in the ewe hypothalamus using [ 3 H]diprenorphine ([ 3 H]DIP) as the radioligand. [ 3 H]DIP binding to hypothalamic membrane preparations was stereospecific, saturable with respect to [ 3 H]DIP concentration, and linear with hypothalamic membrane protein content. Scatchard analysis revealed a single class of binding sites