Murine hybridoma monoclonal antibodies against insulin: Cross-reactivity with insulins of three species and blocking of insulin binding to its receptor

Abstract Six hybridoma cell lines secreting monoclonal antibodies against pig insulin and cross-reacting with human and bovine insulins were obtained. Five of these monoclonal antibodies were IgG1, κ, one IgG2b, κ; their pl values were in the range of pH 6.3–7.4 and dissociation constants of the insulin antibody complexes were 0.3−2 × 10 8 mol/l, as determined by an immunoradiometric inhibition assay. All of these antibodies reacted with sterically closely related determinants and blocked the binding of 125 I-pig insulin to the receptor on human MOLT-4 cell line.