Segregation population selection for genetic linkage maps in Saccharum spontaneum L.

【Objective】The optimal segregation population for genetic linkage map in Saccharum spontaneum L. was constructed to provide theoretical references for follow-up construction of genetic linkage maps, gene mapping and genetic breeding. 【Method】Based on double pseudo-testcross format theory, Saccharum spontaneum L. clones with more genetic divergence at the DNA level and extremely significant difference on the sugar content and tillering rate were used for hybrid access to 3 different cross combinations. The tillering rate and sucrose content of the three candidate mapping populations were analyzed for their variation, normal distribution fitting and molecule polymorphism. 【Result】The tillering rate of combination 3(GXS85-12×GXS87-16)and 13(Yunge①×Tianlin)showed skew distribution, while combination 5 showed approximating normal distribution. The sugar content of combination 3 and 5(GXS85-12×GXS87-16)showed skew distribution, while combination 13 showed approximating normal distribution. On molecular polymorphism level, the parents combinations genetic similarity coefficient of combination 5 with higher variable amplitude was slightly higher than that of combination 3 and 13, but the offspring average genetic similarity coefficient(GS) was lower than the other two. 【Conclusion】The combination 5(GXS85-30×GXS87-16,245 strains) had comparatively hereditary stability in tillering rate and sugar content, wide source population, rich molecular polymorphism and high genetic diversity, hence it could be used as segregation population for genetic maps.