Procoagulant constituents from Cordyceps militaris

Abstract Cordyceps militaris , belongs to Clavicipitaceae family, was investigated for its chemical compounds, and six compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20 and recrystallization, and their structures were elucidated by spectral techniques and physicochemical properties as ergosterol ( 1 ), adenosine ( 2 ), cordycepin ( 3 ), ergosterol peroxide ( 4 ), tetracosanoic acid 2,3-dihydroxypropylester ( 5 ), mannitol ( 6 ). Procoagulant activity was screened by assaying the activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT) and fibrinogen (FIB) in vitro . The results indicated that ergosterol, adenosine, cordycepin, ergosterol peroxide and mannitol showed strong procoagulant activity.