DETECTION OF THE STATE OF THROMBOTIC READINESS IN RATS BY ONE-TIME SUPRATHRESHOLD LOAD OF VARIOUS DURATION BY MEANS OF THROMBOELASTOGRAPHY

Abstract. Introduction. Suprathreshold physical load causing the state of distress in the organism can lead to the damage of various organs and systems including the hemostatic system. A topical integral method of the hemostatic system assessment is thromboelastography. Research objective – to evaluate the state of the hemostatic system by one-time suprathreshold load of various duration by means of thromboelastography. Methods. Experimental groups of rats were exposed to 4-hour and 8-hour physical load in the form of imposed running on a moving platform with the speed of 6-8 m/min. Immediately after the one-time physical load, the blood was examined by the thrombelastograph in the Natem regime for 35 minutes. Results. 4-hour physical load caused reduction of coagulation time (CT), increase of the alpha angle and of the maximum clot firmness (MCF). After the 8-hour load, the thrombelastograph registered the reduction of coagulation time (CT), the increase of the alpha angle, the decrease of the maximum clot firmness (MCF) and reduction of the maximum clot lysis (ML). Conclusions. 4-hour physical load in rats results in partial activation of the hemostatic system without changing the fibrinolytic activity of blood plasm. The revealed changes of thromboelastography parameters indicate high risk of development the state of thrombotic readiness. 8-hour physical load causes the shift of the hemostatic system parameters in rats towards the intensification of clot formation: hypercoagulation, fibrinogen and thrombocytes consumption, suppression of fibrinolysis. The combination of revealed thromboelastogram parameter changes corresponds to the development of thrombotic readiness.