THE RELATIONSHIP OF ANTI-COLD-DRUG AND CLEF LIP AND PALATE: AN EXPERIMENTAL STUDY IN MICE

ve To explore whether the anti-cold-drug produce cleft lip and palate of mouse and to assess the affection of anti-cold-drug acting on the development level of the mouse embryo. Methods Two virgin female was mated overnight with a male mouse of proven fertility. The day on which a vaginal plug was found was designated as day 0 of pregnancy . Dispart pregnant mice to 12 groups randomly, 20 pergnant mice in every group. Mix the anti-cold-drugs into liquid with 0.5% CMC. During the 6th-15th of gestation, the pregnant mice were irrigated with the mixed liquid. Intraperitoneal injection of dexamethasone were given at the 11th and 12th day in the positive control group. On day 18, pregnant females were killed by cervical dislocation. The fetuses were externally examined macroscopically and stereomicrosco-pically . Results No cleft lip and palate were discovered in the control group and anti-cold-drugs experimental groups; There were limbs malformation in the Contac (high). In all anti-cold-drugs experiment groups the fetus height and trail length were significantly shorter than that of the control (P 0.01). In the positive, Shengji (low), Contac (low), Suxiaoshangfeng (low), Suxiaoshangfeng (high), Ganmaotong (low) and Ganmaotong (high), the weight of fetuses were lighter than that of the control (P 0.05) .Conclusion It suggested that anti-cold-drug do not directly result in CLP during pregnancy, but it could induce mouse fetus's developmental retardation and maybe induce congenital limbs malformation of mouse fetus.