Isolation of polymorphic DNA microsatellites in the common sole Solea vulgaris

A microsatellite-enriched genomic library was obtained from the common sole, Solea vulgaris , and seven polymorphic dinucleotide markers were successfully optimized. These markers showed levels of expected heterozygosity ranging from 0.55 to 0.83 and allele number ranging from eight to 12. Three of these markers were also found to successfully amplify in the closely related Solea senegalensis and can be employed to define population genetic structure of the two Solea species and for inferring stock origins.