Establishment and evaluation of transgenic models of Alzheimer's disease with senescence accelerated background

Method In the experiments, APP/PS1 and tau transgenic mice of C57BL/6J background were interbred with SAMP8 mice for five generations. Gene identification of filial generation was tested by PCR. The grading score system developed by Takeda T et al was used for evaluation of the degree of senescence in mice. The agerelated decline of learning and memory ability was analyzed using passive avoidance shuttle box and Morris water maze tasks. Immunofluorescence study was performed to ascertain the presence of b-amyloid (Ab) and tau pathology in AD mouse models by using b-amyloid antibody and specific phosphorylated tau antibody at Ser404.