Secretion of Saliva in X-Irradiated Rat Submandibular Glands

Abstract Takagi, K., Yamaguchi, K., Sakurai, T., Asari, T., Hashimoto, K. and Terakawa, S. Secretion of Saliva in X-Irradiated Rat Submandibular Glands. Radiat. Res. 159, 351–360 (2003). The mechanism of radiation-induced dysfunction in rat submandibular glands was investigated at the cellular level. After X irradiation (single dose, 15 Gy), a vacuolation in the acinar cells or an enlargement of the acinar lumen was observed as a typical morphological change for 2 weeks. As observed using a video-enhanced contrast differential interference contrast (VEC-DIC) microscope, exocytosis and shrinkage of the acinar cells induced by application of pilocarpine (100 μM) were markedly suppressed for 5 days and then recovered to 80% of the control levels. Using an immunohistochemical method, no significant change was observed in amylase distribution, but a marked loss of aquaporin 5 was found in the acinar cells after the irradiation. The extent and time course of pilocarpine-induced mobilization of intracellular Ca2...