Reduction of hexa(sulfobutyl)fullerence on oxidative injury by xanthine and xanthine oxidase in isolated rat lungs.

The present study was undertaken to evaluate whether some fullerenols could effectively reduce direct damages of free radicals produced by xanthine/xanthine oxidase (X/XO) in isolated rat lungs.Female Wistar rats (205 +/- 4 g) were used in studies in pulmonary vascular response to the challenge of xanthine/xanthine oxidase by an isolated-perfused lung method. Free radicals were determined by chemiluminescence (CL) to confirm the release of free radicals after X/XO treatment. The CL count in the lung perfusate was 737 +/- 213 (CL/10 sec); 5 min and 45 min after X/XO administration, the CL counts were 3,778 +/- 425 (CL/10 sec) and 1,183 +/- 193 (CL/10 sec), respectively. Challenge with X/XO caused a mild but significant increase in pulmonary arterial pressure (P(pa)) and a marked increase of filtration coefficient (K(fc)). The pretreatment of Hexa (sulfobutyl) fullerence antioxidant, K(fc) became insignificantly increased in pretreated lungs. In conclusion, We found that hexa(sulfobutyl) fullerene, but not Co60(glucosamine)6, nor superoxide dismutase could attenuate the oxidative stress, judged from the attenuated increase in pulmonary filtration coefficient after challenge.