Immunocytochemical and Biochemical Studies of Glutathione-Peroxidase (GSH-PO) in Rat Gastric Parietal Cells

Histamine as an acid secretagogue and H2-blocker (roxatidine acetate hydrochloride) and a proton pump inhibitor (Omeprazole) as acid secretion inhibitors, in single and repeated doses, were administered to investigate associated lipid peroxidation in rat gastric parietal cells. Methods used included immunohistocytochemical localization and biochemical enzyme activity of glutathione-peroxidase, an enzyme that effectively reduces lipid peroxides. No changes in lipid peroxidation, in response to the H2-blocker, were observed, however its accumulation in the vicinity of vacuole-like structures was specific to proton pump inhibition. This finding suggests that lipid peroxidation occurs in local parietal cells.