RbcL sequence analysis of Lonicera in the South of Zhejiang province

Objective: To identify different Lonicera in the South of Zhejiang province by ribulose 1,5-bisphosphate carboxylase large(rbcL) gene analysis.Methods: The primers designed by Primer Premier 5.0 to amplify the rbcL.ClustalX sequenced the rbcL gene.MEGA computed the value of transitional pairs and transversional pairs.The system diagram of genetic relationship was constructed by Neighbor-Joining and Maximun Parsimony,the out-group was Heptacodium miconioides.Bootstrap evaluated reliability of diagram.Results: 1 143～1 167 bp nucleotide were sequenced in rbcL from Lonicra plant,and remaining 1 137 bp after revision,the conserved site,varible site and Parsim-Information site were 1 126 bp,11 bp and 6 bp respectively.The value of the transitional pairs and transversional pairs was 3 and 2.The Si/Sv was 1.6.Both NJ and MP could divide Lonicera into two groups,one was the Lonicera japonica and Lonicera fulvotomentosa,the other was the Lonicera macranthiodes,Lonicera hypoglauca and Lonicera confusa.Conclusion: The rbcl gene plays an important role in identifing the different Lonicera plant.