Klórhexidint tartalmazó, kontrollált hatóanyag-leadást biztosító, szubgingiválisan alkalmazható készítmény keménységének és hatóanyag-leadásának vizsgálata

Antiseptics – besides mechanical debridement – have an important role in the treatment of destructive periodontitis. Subgingivaldrug delivery systems containing chlorhexidine gluconate (CHX) may enhance or be a substitute to non-surgicaltherapy. In this article, we investigate a CHX-containing drug delivery system which is solid at room temperature but softensat body temperature. The examination was focused on the hardness, the drug diffusion profile and the antimicrobialeffectiveness of the formulation. Hardness was analyzed with a texture analyzer equipped with a spherical probe 5 mm indiameter. The investigation was carried out at 25 and 37 °C. The drug diffusion from formulations was investigated for oneweek in PBS solution. The antimicrobial effectiveness of the delivery systems was analyzed on 6 different anaerobic bacterialstrains. Results show that hardness of formulations at 25 °C is around 2000 mN which is suitable for handling andinsertion, whereas at 37 °C in PBS solution, hardness decreases greatly (to 800 mN) which contributes to the accommodationof the delivery systems to the shape of the periodontal pocket, the penetration of gingival crevicular fluid and thedrug diffusion from formulations. Results of the drug diffusion study indicate that 50% of the CHX diffused from the formulationsduring the one-week period and a concentration of 8 μg/mL were maintained in the acceptor phase after 24 hours.Formulations effectively inhibited the growth of anaerobic bacteria for at least one week and showed similar effectivenessto formulations containing amoxicillin or the combination of metronidazole and zinc hyaluronate. Our results suggest thatthis delivery system alone or in combination with mechanical debridement may be effective in the treatment of periodontitis.