Genetic association analysis of the adiponectin polymorphisms in type 2 diabetes with and without complications

Background: Adiponectin gene polymorphisms are associated with adiponectin plasma levels, BMI, insulin sensitivity and type2 diabetes. This study was carried out to examine the possible association of adiponectin gene polymorphisms at positions +45T/G and –11391G/A and type2 diabetes in an Iranian population. Methods: Type 2 diabetic patients (N=244) were recruited from diabetes clinic in Rafsanjan, South-east of Iran. Healthy control subjects (N=99) were recruited from the same area. DNA was extracted using salting out method Results: No significant association was found between adiponectin gene polymorphisms +45T/G and –11391G/A and diabetes in our study. In addition, no significant association was found between these polymorphisms and diabetic retinopathy, nephropathy and neuropathy. Linkage disequilibrium (LD) analysis showed no significant LD between these two polymorphisms also no significant differences for LD was found between cases and controls . Conclusion: Our findings confirm previous data reporting the lack of association between +45T/G polymorphism in adiponectin gene and development of diabetic neuropathy, retinopathy and nephropathy. Moreover, our data showed no association between -11391 polymorphism and type 2 diabetes .